Related Experiment Videos
Cloning, expression, and nucleotide sequence of a mutant glgC gene from Escherichia coli B
1Department of Biochemistry, Michigan State University, East Lansing 48824.
Journal of Bacteriology
|July 1, 1992
Abstract:
A mutant glgC gene contained in a 10.9-kb PstI fragment was cloned from the Escherichia coli B strain SG5 via colony hybridization by using a wild-type glgC probe. The altered allosteric properties of the expressed ADPglucose synthetase were found to result from the conversion of proline to serine at amino acid residue 295.