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A serotype 10 human rotavirus
Insights
Genome rearrangements in rotaviruses from an immunodeficient child led to the first human serotype 10 isolates. These findings were confirmed using advanced immunological and genetic sequencing techniques.
Area of Science:
- Virology
- Immunology
- Genetics
Background:
- Rotaviruses are a leading cause of severe diarrheal disease in infants and young children worldwide.
- Genome rearrangements in viruses can lead to novel strains with altered pathogenic properties.
- Previous studies had not identified human rotavirus isolates of serotype 10.
Purpose of the Study:
- To identify and characterize novel rotavirus serotypes from a chronically infected immunodeficient child.
- To confirm the serotype of newly isolated human rotaviruses using immunological and molecular methods.
Main Methods:
- Direct enzyme-linked immunosorbent assay (ELISA) for rotavirus detection.
- Virus neutralization assays using serotype-specific monoclonal antibodies.
- Sequence analysis of the VP7 gene for genetic confirmation.
Main Results:
- Rotaviruses with genome rearrangements were isolated from a chronically infected immunodeficient child.
- These isolates were confirmed as human rotavirus serotype 10 through ELISA and neutralization assays.
- Sequence analysis of the VP7 gene showed 96% amino acid homology to the bovine serotype 10 isolate B223, further confirming the serotype.
Conclusions:
- The study reports the first recognized human isolates of rotavirus serotype 10.
- Genome rearrangements played a role in the emergence of this novel human rotavirus serotype.
- The findings expand our understanding of rotavirus diversity and evolution in humans.
Abstract:
Rotaviruses with genome rearrangements isolated from a chronically infected immunodeficient child (F. Hundley, M. McIntyre, B. Clark, G. Beards, D. Wood, I. Chrystie, and U. Desselberger, J. Virol 61:3365-3372, 1987) are the first recognized human isolates of serotype 10. This was shown by both a direct enzyme-linked immunosorbent assay and virus neutralization assays using serotype specific monoclonal antibodies. The serotype was confirmed by sequence analysis of the gene encoding VP7, which revealed a 96% amino acid homology to the bovine serotype 10 isolate B223.