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cAMP-regulated chloride currents in CHO cells.

A W Mangel¹, S Basavappa, J R Raymond

¹Division of Gastroenterology, Duke University Medical Center, Durham, NC 27710.

Biochemical and Biophysical Research Communications

|July 15, 1992

Summary

Elevated cyclic adenosine monophosphate (cAMP) levels activate chloride channels in Chinese hamster ovary (CHO) cells. This increases membrane chloride permeability, particularly at higher cAMP concentrations.

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Area of Science:

Cell biology
Ion channel physiology
Molecular pharmacology

Background:

Chinese hamster ovary (CHO) cells are widely used in biological research.
Cyclic adenosine monophosphate (cAMP) is a crucial second messenger involved in various cellular processes.
Understanding ion channel regulation is vital for cell function.

Purpose of the Study:

To investigate if increased cAMP levels enhance chloride permeability in native CHO cells.
To characterize the nature of cAMP-regulated chloride conductance pathways.

Main Methods:

Measurement of whole-cell chloride currents using electrophysiology.
Quantification of 125I and 36Cl efflux to assess ion permeability.
Application of forskolin to elevate intracellular cAMP levels.

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Main Results:

A significant increase in whole-cell chloride currents and 125I/36Cl efflux was observed at 100 microM forskolin.
Forskolin-activated currents exhibited linear current-voltage relationships.
Reversal potentials confirmed the chloride-selective nature of the forskolin-induced currents.

Conclusions:

Native CHO cells possess cAMP-regulated chloride conductance pathways.
These pathways require substantial elevations in intracellular cAMP to become apparent.
The findings contribute to understanding cAMP signaling in ion transport.