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Updated: Aug 18, 2026

MicroRNA-based Regulation of Picornavirus Tropism
Published on: February 6, 2017
Codon context effect in virus translational readthrough. A study in vitro of the determinants of TMV and Mo-MuLV
R P Valle1, G Drugeon, M D Devignes-Morch
1Institut Jacques Monod Paris, France.
Abstract:
To assess the role of codon context on the efficiency of eukaryotic suppression of termination codons, we have compared, in a rabbit cell-free translation system, the readthrough efficiency related to two synthetic transcripts differing by the codon context around an amber codon. The codon contexts are derived from tobacco mosaic virus (TMV) and Moloney murine leukemia virus (Mo-MuLV) RNAs. The Mo-MuLV-like codon context does not promote suppression. Substituting TMV-derived triplets in the Mo-MuLV-like codon context shows that the two codons downstream from the TMV UAG signal are important determinants of suppression, as recently demonstrated in vivo.
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