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Experimental in vivo generation of intertypic recombinant strains of HSV in the mouse
D L Yirrell1, C E Rogers, W A Blyth
1Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, U.K.
Abstract:
The primary replication of one strain of HSV was generally unaffected by the simultaneous inoculation of another strain either at the same site or at a different site within the same dermatome. Exceptions to this were the result of the generation of intertypic recombinants which were readily isolated only from sensory ganglia 5 and 6 days after inoculation with a mixture of HSV-1 and HSV-2 and from explant culture of the resultant latently infected ganglia. By restriction enzyme analysis the majority of the recombinant strains from primary infection were characterized as HSV 1; all those from latently infected ganglia were characterized as type 2.
Insights
Simultaneous herpes simplex virus (HSV) infections rarely interfere with primary replication. However, intertypic recombinants emerge, particularly in latently infected ganglia, showing a shift from HSV-1 to HSV-2 characteristics.
Area of Science:
- Virology
- Molecular Biology
- Immunology
Background:
- Herpes simplex virus (HSV) infections are common, with two main types: HSV-1 and HSV-2.
- Understanding viral interactions during coinfection is crucial for disease management.
Purpose of the Study:
- To investigate the impact of simultaneous inoculation of different HSV strains on primary replication.
- To identify and characterize any intertypic recombinant strains formed during coinfection.
Main Methods:
- Simultaneous inoculation of different HSV strains in vivo.
- Isolation and characterization of viral strains from sensory ganglia.
- Restriction enzyme analysis to determine recombinant strain types.
Main Results:
- Primary replication of one HSV strain was generally unaffected by coinfection.
- Intertypic recombinant strains were isolated from sensory ganglia 5-6 days post-inoculation.
- Recombinants from primary infection were predominantly HSV-1, while those from latently infected ganglia were predominantly HSV-2.
Conclusions:
- Coinfection with different HSV strains can lead to the generation of intertypic recombinants.
- Latent infection appears to favor the selection or generation of HSV-2-like recombinants.