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Tcrb-V3+ T-cell deletion and a mouse mammary tumor provirus, Mtv-27
K Tomonari1, S Fairchild, O A Rosenwasser
1Transplantation Biology Section, MRC Clinical Research Centre, Harrow, UK.
Abstract:
Genes encoding superantigens which delete Tcrb-V3+ T cells co-segregate with mouse mammary tumor proviruses (Mtv), Mtv-1, Mtv-3, Mtv-6, Mtv-13, and Mtv-44. We have examined percentages of Tcrb-V3+ T cells and Mtv integrations in [(B10 x NZB)F1 x B10.BR] mice, and show that Mtv-27 as well as Mtv-3 from NZB mice co-segregate with genes encoding deletion ligands for Tcrb-V3+ T cells without recombination.
Insights
Mouse mammary tumor viruses (Mtv) genes linked to T-cell deletion were identified. Specific Mtv elements, including Mtv-27 and Mtv-3, were found to co-segregate with T-cell deletion ligands.
Area of Science:
- Immunology
- Virology
- Genetics
Background:
- Superantigens encoded by mouse mammary tumor proviruses (Mtv) can eliminate specific T cells.
- Previous studies linked certain Mtv elements to the deletion of Tcrb-V3+ T cells.
Purpose of the Study:
- To investigate the co-segregation of Mtv integrations with genes responsible for Tcrb-V3+ T cell deletion.
- To identify specific Mtv elements associated with T cell deletion ligands.
Main Methods:
- Analysis of Tcrb-V3+ T cell percentages in specific mouse strains.
- Examination of Mtv proviral integrations in [(B10 x NZB)F1 x B10.BR] mice.
- Genetic analysis to determine co-segregation patterns.
Main Results:
- Genes encoding superantigens that delete Tcrb-V3+ T cells co-segregate with Mtv-1, Mtv-3, Mtv-6, Mtv-13, and Mtv-44.
- Mtv-27 and Mtv-3 from NZB mice were found to co-segregate with genes encoding deletion ligands for Tcrb-V3+ T cells.
- This co-segregation occurred without evidence of recombination.
Conclusions:
- Specific Mtv elements are genetically linked to the deletion of Tcrb-V3+ T cells.
- Mtv-27 and Mtv-3 play a role in T cell repertoire shaping through ligand-mediated deletion.