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Related Experiment Videos

Thyroid hormone modulates apolipoprotein B gene expression in HepG2 cells.

A Theriault1, G Ogbonna, K Adeli

  • 1Department of Chemistry and Biochemistry, University of Windsor, Ontario, Canada.

Biochemical and Biophysical Research Communications
|July 31, 1992
PubMed
Summary

Thyroid hormone significantly boosts apolipoprotein B (ApoB) production and gene expression in liver cells. This hormonal effect occurs at both transcriptional and posttranscriptional levels, impacting ApoB levels.

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Area of Science:

  • Endocrinology
  • Molecular Biology
  • Hepatology

Background:

  • Apolipoprotein B (ApoB) is crucial for lipoprotein metabolism and cardiovascular health.
  • Thyroid hormones play a vital role in regulating various metabolic processes, including lipid metabolism.

Purpose of the Study:

  • To investigate the effect of thyroid hormone on apolipoprotein B (ApoB) gene expression in HepG2 cells.
  • To elucidate the mechanisms by which thyroid hormone modulates ApoB production.

Main Methods:

  • Long-term cultures of HepG2 cells exposed to triiodothyronine.
  • Measurement of ApoB secretion rates.
  • Quantification of ApoB mRNA levels using slot- and Northern-blot analysis.
  • Assessment of ApoB synthesis rates via in vivo pulse-chase and in vitro translation studies.

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Main Results:

  • Thyroid hormone significantly increased ApoB secretion in a dose-dependent manner.
  • Triiodothyronine elevated ApoB mRNA levels by 25-36%.
  • ApoB synthesis rate increased by 54.5-61%, with a concurrent 30% increase in secretion, suggesting intracellular degradation.

Conclusions:

  • Thyroid hormone stimulates ApoB gene expression at both transcriptional and posttranscriptional levels.
  • The findings highlight a significant role for thyroid hormone in regulating ApoB metabolism.
  • Potential implications for understanding lipid disorders and cardiovascular disease risk.