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Type VI collagen microfibrils: evidence for a structural association with hyaluronan
C M Kielty1, S P Whittaker, M E Grant
1Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, England.
The Journal of Cell Biology
|August 1, 1992
Summary
Type VI collagen forms microfibrils associated with hyaluronan in connective tissues. This interaction is crucial for tissue growth and remodeling.
Area of Science:
- Biochemistry
- Cell Biology
- Connective Tissue Research
Background:
- Type VI collagen is a major structural protein in connective tissues.
- Its precise role and interactions within the extracellular matrix are not fully understood.
Purpose of the Study:
- To investigate the structural relationship between type VI collagen and hyaluronan.
- To elucidate the functional significance of this association in connective tissues.
Main Methods:
- Isolation of type VI collagen from fetal bovine skin using bacterial collagenase digestion and gel filtration.
- Analysis of microfibrillar structure using rotary shadowing electron microscopy.
- Assessment of hyaluronan interaction and disruption/repolymerization of collagen VI using hyaluronidase and hyaluronan.
Main Results:
- Type VI collagen was isolated as intact microfibrillar arrays.
- These microfibrils were found to be associated with hyaluronan.
- Hyaluronidase treatment disrupted microfibrils, while hyaluronan facilitated partial repolymerization, indicating a specific binding interaction.
Conclusions:
- A specific structural and functional relationship exists between type VI collagen and hyaluronan.
- This association is vital for the growth and remodeling of connective tissues.