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MPO activity and generation of active O2 species in leukocytes from poorly controlled diabetic patients
1Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Objective:
This study was undertaken to determine which part of ROI generation is reduced in the neutrophils from patients with NIDDM.
Research Design And Methods:
Superoxide anion (O2-) production, LDCL activity in response to opsonized zymosan, and MPO activity were measured in leukocytes of poorly controlled NIDDM patients (FBG greater than 8.89 mM, HbA1 greater than 10%).
Results:
In diabetic subjects, both O2- production and LDCL activity assessed with initial slope gradient and peak value were significantly reduced. MPO activity was also decreased in diabetic subjects, and there was a significant correlation between HbA1 levels and MPO activity of diabetic subjects.
Conclusions:
This study demonstrated that every step in leukocyte ROI generation should be reduced in the leukocytes from poorly controlled diabetic patients.