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Direct expression of active spinach glycolate oxidase in Escherichia coli
P Macheroux1, S B Mulrooney, C H Williams
1Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor.
Biochimica Et Biophysica Acta
|August 27, 1992
Abstract:
Spinach glycolate oxidase (GAO) was expressed in Escherichia coli using the T7 RNA polymerase promotor. The enzyme accounts for approx. 1% of the soluble protein fraction and is expressed as a soluble and active enzyme. Comparison with GAO expressed in Saccharomyces cerevisiae (Macheroux, P., Massey, V., Thiele, D.J. and Volokita, M. (1991) Biochemistry 30, 4612-4619) showed that the GAO expressed in E. coli has identical physico-chemical features to the wild-type enzyme, but is expressed at a level approx. 15-fold higher than in the yeast system.