Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Insulin-like growth factors decrease oxygen-regulated erythropoietin production by human hepatoma cells (Hep G2).

H Scholz1, W Baier, P Ratcliffe

  • 1Physiologisches Institut, Universität Zürich, Switzerland.

The American Journal of Physiology
|August 1, 1992
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Columbia spotted frogs (Rana luteiventris) have characteristic skin microbiota that may be shaped by cutaneous skin peptides and the environment.

FEMS microbiology ecology·2020
Same author

Analysis of the calcium paradox of renin secretion.

American journal of physiology. Renal physiology·2018
Same author

CD73/5'-ecto-nucleotidase acts as a regulatory factor in osteo-/chondrogenic differentiation of mechanically stimulated mesenchymal stromal cells.

European cells & materials·2013
Same author

Presymptomatic genetic testing in CADASIL.

Journal of neurology·2012
Same author

Regulation of renin release by local and systemic factors.

Reviews of physiology, biochemistry and pharmacology·2011
Same author

Cytomegalovirus-specific regulatory and effector T cells share TCR clonality--possible relation to repetitive CMV infections.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2011

Insulin-like growth factors (IGFs) and insulin reduce erythropoietin (EPO) production in Hep G2 cells. These hormones inhibit EPO formation stimulated by low oxygen or cobalt, primarily through the IGF-I receptor.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Cell Biology

Background:

  • Erythropoietin (EPO) is a key hormone regulating red blood cell production.
  • Oxygen levels and certain chemical factors significantly influence EPO synthesis.
  • The role of growth factors and insulin in EPO regulation requires further elucidation.

Purpose of the Study:

  • To investigate the impact of insulin-like growth factors (IGFs) and insulin on EPO production in human hepatoma (Hep G2) cells.
  • To determine the specific mechanisms and receptors involved in the regulation of EPO synthesis by IGFs and insulin.

Main Methods:

  • Hep G2 cells were cultured under varying oxygen conditions (normoxia and hypoxia) and treated with cobalt chloride.
  • EPO production was quantified, and EPO mRNA levels were assessed.

Related Experiment Videos

  • Cellular responses to IGF-I, IGF-II, and insulin, including receptor binding and metabolic activity, were measured.

    • Main Results:

    • Hypoxia and cobalt chloride significantly increased EPO production in Hep G2 cells.
    • IGF-I, IGF-II, and insulin demonstrated a concentration-dependent inhibition of EPO production.
    • IGF-I also stimulated protein synthesis, DNA incorporation, and glycogen synthesis, and bound to the IGF-I receptor with high affinity.

    Conclusions:

    • IGFs and insulin negatively regulate oxygen-dependent EPO production in Hep G2 cells.
    • The inhibitory effects of IGFs and insulin on EPO formation are likely mediated via the IGF-I receptor.
    • These findings highlight a complex interplay between growth factors, insulin, and the EPO system.