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Affinity chromatographic purification of bovine lung endothelin receptor using biotinylated endothelin and
H Hagiwara1, T Nagasawa, K M Lodhi
1Department of Biological Sciences, Tokyo Institute of Technology, Japan.
Journal of Chromatography
|April 24, 1992
Abstract:
Endothelin receptor was purified from bovine lung by affinity chromatography using biotinylated endothelin and avidin-agarose. Endothelin was biotinylated with sulphosuccinimidyl 2-(biotinamido)ethyl-1,3-dithiopropionate, a reactive form of biotin with a cleavable spacer arm containing a disulphide bond designed for a simple elution by thiols. Starting from 3.5 kg of bovine lung, about 200 micrograms of pure receptor were obtained.