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Related Experiment Videos

Endothelin-specific antibodies decrease blood pressure and increase glomerular filtration rate and renal plasma flow

A Ohno1, M Naruse, S Kato

  • 1Department of Medicine, Tokyo Women's Medical College, Japan.

Journal of Hypertension
|August 1, 1992
PubMed
Summary

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Neutralizing endogenous endothelin with specific antibodies significantly lowered blood pressure and improved renal function in spontaneously hypertensive rats (SHR), indicating endothelin

Area of Science:

  • Cardiovascular Physiology
  • Renal Physiology
  • Endocrinology

Background:

  • Endogenous endothelin's role in blood pressure regulation and renal hemodynamics is not fully understood.
  • Spontaneously hypertensive rats (SHR) serve as a model for studying hypertension-related pathophysiology.

Purpose of the Study:

  • To investigate the pathophysiologic significance of endogenous endothelin in controlling blood pressure and renal hemodynamics in SHR.
  • To quantify the contribution of endothelin to in vivo blood pressure and renal function regulation.

Main Methods:

  • Passive immunization using endothelin-specific antibodies to neutralize endogenous endothelin.
  • Intravenous administration of antibodies into anesthetized SHR and Wistar-Kyoto (WKY) rats.
  • Assessment of blood pressure, renal plasma flow, and glomerular filtration rate; radioimmunoassay for plasma endothelin levels.

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Main Results:

  • Antibody infusion in SHR decreased mean arterial pressure by ~10% and renal vascular resistance by ~35%.
  • Glomerular filtration rate and renal plasma flow increased by ~50% in SHR after antibody infusion.
  • No significant changes were observed in WKY rats or with normal serum in SHR, and SHR had lower baseline endothelin levels than WKY rats.

Conclusions:

  • Endothelin plays a significant role in modulating systemic blood pressure in SHR.
  • Endogenous endothelin is crucial for regulating renal function in spontaneously hypertensive rats.