Immunologic and chemical studies on mucopolysaccharide derived from murine lymphosarcoma

Mucopolysaccharide was prepared from media in which the Gardner's lymphosarcoma cells were cultured. Multiple intraperitoneal injections of the preparation into syngeneic mice resulted in enhancement of the tumors which were inoculated prior to the injections. Sera of these hosts contained antibodies reacting with antigens of the outer layer of the tumor cells. Chemical analyses of the preparation showed that the mucopolysaccharide is composed of hexosamine, uronic, acid, hexose, and proteins. The mucopolysaccharide was shown to possess unique migration rate on electrophoresis and thin layer chromatography.