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Sequential tubular cell and basement membrane changes in polycystic kidney disease
F A Carone1, S Nakamura, P Punyarit
1Department of Pathology, Northwestern University Medical School, Chicago, IL 60611-3008.
Journal of the American Society of Nephrology : JASN
|August 1, 1992
Summary
In polycystic kidney disease models, initial cyst formation correlates with basement membrane changes. Cell proliferation and altered Na,K-ATPase location occur after cyst appearance.
Area of Science:
- Nephrology
- Cell Biology
- Pathology
Background:
- Renal cyst development is linked to basement membrane (BM) changes, cell proliferation, and fluid accumulation.
- Altered Na,K-ATPase localization is implicated in cystogenesis.
Purpose of the Study:
- To investigate the temporal relationship between BM changes, cell proliferation, and Na,K-ATPase localization during renal cyst development.
- To assess these events in a rat model using Phenol II treatment.
Main Methods:
- Daily assessment of collecting tubules (CT) in Phenol II-treated rats.
- Evaluation of BM thickness, composition (heparan sulfate proteoglycan, fibronectin), cell proliferation (3H-thymidine incorporation), and Na,K-ATPase localization (immunohistochemistry).
Main Results:
- Initial cyst formation (Day 1) showed thickened BM with altered composition.
- Significant cell proliferation began on Day 2, involving various cell types.
- Altered Na,K-ATPase localization was detected after Day 2, with cytoplasmic and rare apical membrane presence.
- Reversibility of changes observed after Phenol II removal.
Conclusions:
- In this polycystic kidney disease model, cyst formation is initially driven by BM structural changes.
- Cell proliferation and abnormal Na,K-ATPase localization follow the initial cyst appearance.
- These findings clarify the sequence of events in polycystic kidney disease pathogenesis.