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Vasoactive intestinal peptide enhances dopamine accumulation in primary cell culture of rat hypothalamus
K Kadowaki1, K Koike, K Hirota
1Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Endocrinologia Japonica
|April 1, 1992
Abstract:
The effect of vasoactive intestinal peptide (VIP) and PHI-27 on dopamine accumulation in cultured rat hypothalamic cells was investigated. VIP enhanced [3H]dopamine accumulation dose dependently. This effect was significant at 10(-8)-10(-5) M VIP with a concomitant increase in intracellular cyclic AMP (cAMP), and reached its plateau level at 10(-6) M VIP. VIP increased [3H]dopamine accumulation significantly within 15 min. PHI-27 and dibutyryl cAMP ((Bu)2-cAMP) also enhanced [3H]dopamine accumulation. These results suggest that VIP enhances dopamine accumulation in hypothalamic cells by increasing intracellular cAMP.