In vitro study of the effect of adenosine on frog adrenocortical cells

Previous reports have shown that adenosine in rat inhibits both spontaneous and ACTH-induced release of corticosteroids through activation of adenosine A1 receptors. In the present study, we have investigated the possible effect of adenosine in the secretion of corticosteroids in amphibians using a perfusion technique for frog adrenocortical slices. Infusion of adenosine, at concentrations ranging from 10(-7) to 10(-4) M, had no effect on the basal output of corticosterone and aldosterone by frog interrenal cells. Similarly, adenosine did not affect the response of frog adrenocortical slices to ACTH, vasoactive intestinal peptide, or angiotensin II. The stable adenosine A1 receptor agonist N6-phenylisopropyl adenosine (PIA) was also totally devoid of effect on the spontaneous or ACTH-induced release of corticosteroids. These results show that in amphibians, adenosine does not modulate adrenal steroidogenesis.