Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intergeneric conjugation in Thiobacillus versutus.

D L Read1, L M Toth, K McCann

  • 1Biology Department, University of Massachusetts Dartmouth 02747.

The Journal of Applied Bacteriology
|August 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of obesity on postoperative complications in ulcerative colitis: A systematic review and meta-analysis.

Annals of gastroenterological surgery·2025
Same author

Spontaneous food allergy in Was<sup>-/-</sup> mice occurs independent of FcεRI-mediated mast cell activation.

Allergy·2017
Same author

Improvement of ELISA procedures through simultaneous addition of antigen and detection antibody and elimination of washing steps.

Journal of immunoassay & immunochemistry·2017
Same author

Approach to evaluation of fever in ambulatory cancer patients receiving chemotherapy: A systematic review.

Cancer treatment reviews·2016
Same author

Approach to fever assessment in ambulatory cancer patients receiving chemotherapy: a clinical practice guideline.

Current oncology (Toronto, Ont.)·2016
Same author

We really are open all hours in Skegness!

Nursing standard (Royal College of Nursing (Great Britain) : 1987)·2016
Same journal

Note: isolation, characterization and epidemiology of Yersinia enterocolitica from humans and animals.

The Journal of applied bacteriology·1996
Same journal

Isolation, partial characterization and mode of action of acidocin J1229, a bacteriocin produced by Lactobacillus acidophilus JCM 1229.

The Journal of applied bacteriology·1996
Same journal

The use of selective and differential agars in the isolation of Escherichia coli O157 from dairy herds.

The Journal of applied bacteriology·1996
Same journal

A rat model of infection by Salmonella typhimurium or Salm. enteritidis.

The Journal of applied bacteriology·1996
Same journal

The incidence and level of Listeria monocytogenes contamination of food sources at primary production and initial processing.

The Journal of applied bacteriology·1996
Same journal

Heat-stable serotyping antigens expressed by strains of Campylobacter jejuni are probably capsular and not long-chain lipopolysaccharide.

The Journal of applied bacteriology·1996
See all related articles

Transposon Tn5 successfully conferred kanamycin resistance in Thiobacillus versutus, despite the suicide vector

Area of Science:

  • Microbiology
  • Bacterial Genetics
  • Molecular Biology

Background:

  • Thiobacillus versutus is a bacterium relevant to sulfur oxidation.
  • Gene transfer mechanisms in T. versutus are not fully understood.
  • Transposon mutagenesis is a key tool for bacterial genetics.

Purpose of the Study:

  • To investigate the efficiency of Tn5 transposition in Thiobacillus versutus.
  • To determine if kanamycin resistance in T. versutus exconjugants is due to functional Tn5.
  • To analyze the fate of the suicide vector during gene transfer.

Main Methods:

  • Plate matings between Escherichia coli HB101/pUW965::Tn5 and Thiobacillus versutus.
  • Selection of kanamycin-resistant (KmR) exconjugants.
  • Plasmid DNA analysis using agarose gel electrophoresis.

Related Experiment Videos

  • Hybridization techniques to detect Tn5 presence.

    • Main Results:

    • Efficient transfer of kanamycin resistance (10(-2) to 10(-3) exconjugants per donor).
    • Absence of the suicide vector pUW964 in T. versutus exconjugants.
    • Negative hybridization results for Tn5 presence in exconjugants, despite conferred resistance.

    Conclusions:

    • Tn5 transposition likely occurred in T. versutus, conferring kanamycin resistance.
    • The kanamycin resistance gene from E. coli appears functional in T. versutus.
    • The inability to detect Tn5 via hybridization presents an anomalous finding requiring further investigation.