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Streptococcus mutans serotype c tagatose 6-phosphate pathway gene cluster.

E K Jagusztyn-Krynicka1, J B Hansen, V L Crow

  • 1Department of Microbiology, University of Alabama, Birmingham 35294.

Journal of Bacteriology
|October 1, 1992
PubMed
Summary

Researchers identified genes in Streptococcus mutans responsible for galactose metabolism. These genes encode enzymes for the tagatose 6-phosphate pathway, crucial for sugar utilization.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Streptococcus mutans is a key bacterium in dental caries.
  • Understanding its metabolic pathways, like galactose utilization, is important for controlling its growth and virulence.
  • The tagatose 6-phosphate pathway is a known route for galactose metabolism in some bacteria.

Purpose of the Study:

  • To clone and characterize the genes responsible for galactose utilization in Streptococcus mutans serotype c.
  • To determine the enzymatic activities, gene order, and protein sizes involved in the tagatose 6-phosphate pathway.

Main Methods:

  • Cloning of DNA from Streptococcus mutans into Escherichia coli K-12.
  • Analysis of protein expression in recombinant plasmids using minicells.

Related Experiment Videos

  • Gene mapping and ordering using transposon Tn5 mutagenesis.

    • Main Results:

    • Three enzyme activities for the tagatose 6-phosphate pathway were identified: galactose 6-phosphate isomerase, tagatose 6-phosphate kinase, and tagatose-1,6-bisphosphate aldolase.
    • The genes were localized to a 3.28-kb HindIII DNA fragment.
    • The molecular weights of the subunits/proteins were determined: 14- and 19-kDa for isomerase, 34-kDa for kinase, and 36-kDa for aldolase.

    Conclusions:

    • The cloned DNA fragment from Streptococcus mutans contains the complete genetic information for the tagatose 6-phosphate pathway.
    • The characterized enzymes and their sizes are consistent with those found in other bacterial species like Staphylococcus aureus and Lactococcus lactis.
    • This study provides a molecular basis for understanding galactose metabolism in Streptococcus mutans.