Dentin as inhibitor of bacterial toxicity on pulpal cells in vitro

Many studies have shown that the major cause of pulpal disease is the presence of bacteria or their by-products in the dentinal tubules. The purpose of this investigation was to develop an in vitro model, simulating the pulp chamber, that would permit the study of the transport of bacterial by-products through dentin and their effect on pulpal cells. Human pulpal cells were cultured in a modified Sykes-Moore chamber and exposed through dentin to sonicated extracts of Porphyromonas gingivalis ATCC 33277. The cell response was evaluated with the thymidine incorporation method. The results were compared with the cell response obtained after direct exposure to the same irritant. It was found that dentin significantly restricts the diffusion of bacterial proteins in a 24-h experimental period. The time needed for the first bacterial protein molecule to cross the dentin barrier was 6 h. The "diffusion velocity" of the bacterial proteins was 0.023 microns/s. The proposed model has further applications in biocompatibility and microleakage research.