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Antigenic relationships among human herpesvirus-6 isolates.
B Chandran1, S Tirawatnapong, B Pfeiffer
1Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City.
Journal of Medical Virology
|August 1, 1992
Summary
Human herpesvirus 6 (HHV-6) isolates can be classified into two distinct groups, A and B, based on DNA restriction analysis and antigenic differences. This classification aids in understanding HHV-6 diversity and its implications in various diseases.
Area of Science:
- Virology
- Immunology
- Molecular Biology
Background:
- Human herpesvirus 6 (HHV-6) is a lymphotropic herpesvirus with newly identified prototype isolate GS.
- Subsequent HHV-6 isolates were identified through hybridization to a specific DNA probe.
Purpose of the Study:
- To investigate the antigenic relationships among 15 HHV-6 isolates.
- To determine if HHV-6 isolates can be divided into distinct groups based on antigenic properties.
Main Methods:
- DNA restriction analysis and in vitro tropism studies to initially group isolates.
- Immunoprecipitation using rabbit polyclonal antibodies and 11 monoclonal antibodies against HHV-6(GS) infected cells.
- Serological analysis using sera from 136 healthy adults via immunofluorescence assay.
Main Results:
- HHV-6 isolates were successfully divided into two groups, A and B, consistent with DNA restriction analysis.
- Monoclonal antibody reactivities confirmed the segregation of isolates into group A (including HHV-6(GS)) and group B (including HHV-6(Z-29)).
- While most adult sera showed similar antibody titers against both groups, 19% exhibited differences, and polypeptide analysis revealed variations between group A and B isolates.
Conclusions:
- HHV-6 isolates exhibit antigenic heterogeneity, supporting their classification into two distinct groups.
- These findings contribute to the understanding of HHV-6 diversity and potential variations in immune responses.