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Related Experiment Videos

Structural proteins of equine arteritis virus.

A A de Vries1, E D Chirnside, M C Horzinek

  • 1Department of Infectious Diseases and Immunology, Veterinary Faculty, University of Utrecht, The Netherlands.

Journal of Virology
|November 1, 1992
PubMed
Summary

Equine arteritis virus (EAV) structural proteins, including nucleocapsid (N) and membrane proteins (M, GS, GL), were identified and their genes assigned. The study reveals distinct virion architecture, supporting EAV

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Area of Science:

  • Virology
  • Molecular Biology
  • Protein Chemistry

Background:

  • Equine arteritis virus (EAV) genome organization was previously established, revealing seven open reading frames (ORFs).
  • Understanding the structural proteins and their genetic origins is crucial for classifying EAV and related viruses.

Purpose of the Study:

  • To identify and characterize the structural proteins of Equine arteritis virus (EAV).
  • To assign the genes responsible for the synthesis of these structural proteins.
  • To determine the relative abundance and post-translational modifications of EAV structural proteins.

Main Methods:

  • Analysis of virion composition, including molecular weight determination of nucleocapsid (N) and membrane proteins (M, GS, GL).
  • Use of monospecific antisera and antivirion serum for protein identification.

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  • Expression of individual ORFs to confirm gene assignments for structural proteins.

    • Main Results:

    • EAV virions comprise a 14-kDa nucleocapsid protein (N) and three membrane proteins: M (16 kDa, unglycosylated), GS (25 kDa, N-glycosylated), and GL (30-42 kDa, N-glycosylated).
    • Genes encoding structural proteins were assigned: ORF 7 (N), ORF 6 (M), ORF 5 (GL), and ORF 2 (GS).
    • Proteins N, M, and GL are abundant in virions (molar ratio ~3:2:3), while GS is significantly underrepresented (1-2%). GL exhibits size heterogeneity due to N-acetyllactosamine addition.

    Conclusions:

    • The identified structural proteins and their gene assignments provide a comprehensive understanding of EAV virion composition.
    • The distinct virion architecture of EAV, despite some transcriptional similarities to coronaviruses and toroviruses, supports its unique taxonomic position.
    • Findings contribute to the classification of EAV, lactate dehydrogenase-elevating virus, and simian hemorrhagic fever virus.