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Disulfide bonds are required for Serratia marcescens nuclease activity
1Department of Biochemical and Biophysical Sciences, University of Houston, TX 77204-5934.
Nucleic Acids Research
|October 11, 1992
Abstract:
The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm.