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Effect of pulmonary thromboembolism on circulating neutrophils in mice
M Dikshit1, R Kumari, R C Srimal
1Division of Pharmacology, Central Drug Research Institute, Lucknow, India.
Abstract:
The role of free radical generation and its scavenging enzymes in circulating mice polymorphonuclear leukocytes (PMNLs) has been studied following pulmonary thromboembolism. Levels of malonaldehyde (MDA), 02- radical generation, activity of superoxide dismutase (SOD), catalase (CAT), lactate dehydrogenase (LDH), myeloperoxidase (MPO) and lysozyme were estimated in lysed neutrophil preparations. Activities of SOD and CAT were increased in neutrophils, while animals showed 60 +/- 4% thrombocytopenia. Levels of MDA in PMNLs were also elevated significantly following thrombosis. However, there was no significant change in superoxide radical generation, after thrombotic challenge, in mice neutrophils. The present study provides evidence for the involvement of free radicals in mice pulmonary thromboembolism.
Insights
Pulmonary thromboembolism in mice involves free radicals. Neutrophils showed increased malonaldehyde and scavenging enzyme activity, indicating oxidative stress during this condition.
Area of Science:
- Biomedical Science
- Physiology
- Pathology
Background:
- Pulmonary thromboembolism (PTE) is a significant clinical condition.
- The role of oxidative stress in PTE is not fully understood.
- Polymorphonuclear leukocytes (PMNLs) are implicated in inflammatory responses during PTE.
Purpose of the Study:
- To investigate the generation of free radicals and the activity of scavenging enzymes in circulating mice PMNLs following PTE.
- To assess biochemical markers of oxidative stress and cellular damage in neutrophils.
Main Methods:
- Pulmonary thromboembolism was induced in mice.
- Levels of malonaldehyde (MDA), superoxide radical generation, superoxide dismutase (SOD), catalase (CAT), lactate dehydrogenase (LDH), myeloperoxidase (MPO), and lysozyme were measured in lysed neutrophil preparations.
- Thrombocytopenia was assessed.
Main Results:
- Activities of SOD and CAT were significantly increased in neutrophils post-thromboembolism.
- Malonaldehyde (MDA) levels in PMNLs were also significantly elevated.
- A notable thrombocytopenia (60 +/- 4%) was observed.
- Superoxide radical generation did not show a significant change in mice neutrophils after thrombotic challenge.
Conclusions:
- The study provides evidence for the involvement of free radicals and oxidative stress in mice pulmonary thromboembolism.
- Increased MDA and scavenging enzyme activity in PMNLs suggest a role for neutrophils in the inflammatory response during PTE.