Related Experiment Videos
Hepatitis C virus in peripheral blood mononuclear cells
T T Hsieh1, D S Yao, I S Sheen
1Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Taipei, Taiwan, Republic of China.
American Journal of Clinical Pathology
|October 1, 1992
Summary
Hepatitis C virus RNA is frequently detected in the plasma of patients with chronic hepatitis C. This study also found the virus in a significant portion of their peripheral blood mononuclear cells, suggesting a potential role in disease.
Area of Science:
- Virology
- Hepatology
- Immunology
Background:
- Chronic hepatitis C is a significant global health concern.
- Hepatitis C virus (HCV) infection can lead to serious liver damage.
- The presence and role of HCV in different blood components are not fully understood.
Purpose of the Study:
- To investigate the prevalence of Hepatitis C virus RNA in plasma and peripheral blood mononuclear cells (PBMCs) of patients with chronic hepatitis C.
- To explore the potential significance of HCV detection in PBMCs.
Main Methods:
- RNA extraction from patient plasma and PBMCs.
- Reverse transcription followed by nested PCR amplification for HCV detection.
Main Results:
- HCV RNA was detected in 93.3% of plasma samples.
- HCV RNA was detected in 53.3% of PBMC samples from the same patients.
- A high detection rate of HCV was observed in both plasma and PBMCs.
Conclusions:
- Hepatitis C virus is frequently present in the peripheral blood mononuclear cells of patients with chronic hepatitis C.
- Further research is needed to determine the role of HCV in PBMCs in the pathogenesis of hepatitis C-associated diseases.