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Inosine-containing primers in human papillomavirus detection by polymerase chain reaction
G Novelli1, M Gennarelli, L De Santis
1Department of Public Health and Cell Biology, 2nd University of Rome, Italy.
Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|January 1, 1992
Abstract:
A PCR protocol has been developed using inosine-containing primers for human papilloma virus (HPV) detection. The HPV16 L1 region from CaSki-positive cells was efficiently amplified by a single reaction and directly analyzed by agarose gel electrophoresis. The method was found to be sensitive and reproducible, and it easy to use for HPV typing and screening.