Comparison of three immunoassays for the detection of anti-HHV6

Sera from 96 blood donors were tested for antibody to human herpesvirus 6 by indirect immunofluorescence (IF), circle immunoassay (CIA) and competitive radioimmunoassay (RIA). The correlation between the three assays was good but the CIA and competitive RIA were more sensitive for the detection of HHV6 antibody than indirect IF. The crossreaction of HHV6 antibody with that to the other human herpesviruses was also studied in this blood donor group. No correlation was found between antibody to human herpesvirus 6 by any of the methods described and antibody to any of the other human herpesviruses in these sera.