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Bovine inositol monophosphatase: development of a continuous fluorescence assay of enzyme activity
M G Gore1, P J Greasley, C I Ragan
1Dept. of Biochemistry, School of Biological Sciences, University of Southampton, UK.
Journal of Biochemical and Biophysical Methods
|August 1, 1992
Abstract:
This paper describes a continuous assay for the enzyme inositol monophosphatase which has been developed using a new substrate, the fluorescent compound 4-methylumbelliferyl phosphate. The hydrolysis of the phosphate group from this compound can be readily detected by a resultant large red shift in the emission spectrum from 390-450 nm. The kinetic constants for the enzyme using this new substrate are described.