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Monoclonal antibody to human plasma gelsolin
M Hiyoshi1, Y Terano, N Tatsumi
1Department of Laboratory Medicine, Osaka City University Medical School.
Japanese Journal of Medical Science & Biology
|April 1, 1992
Summary
Researchers developed a new monoclonal antibody (Mab H6B11) targeting human plasma gelsolin. This antibody effectively neutralizes F-actin depolymerization and offers faster assay times for studying gelsolin’s physiological roles.
Area of Science:
- Biochemistry
- Immunology
- Cell Biology
Background:
- Gelsolin is a key protein involved in actin filament dynamics.
- Understanding gelsolin's physiological roles requires specific and efficient detection methods.
Purpose of the Study:
- To produce a novel monoclonal antibody against human plasma gelsolin.
- To characterize the antibody's reactivity and functional activity.
- To assess its utility in biochemical assays.
Main Methods:
- Purification of human plasma gelsolin using column chromatography.
- Production of monoclonal antibody (Mab H6B11) via somatic cell fusion.
- Enzyme-linked immunosorbent assay (ELISA) and F-actin depolymerization assays.
Main Results:
- High purity and active human plasma gelsolin was obtained.
- Mab H6B11 demonstrated dose-dependent reactivity with human plasma and platelet gelsolin.
- The antibody neutralized F-actin depolymerizing activity.
- ELISA reaction times were reduced by one-third compared to a commercial antibody.
Conclusions:
- Mab H6B11 is a potent antibody against human plasma gelsolin.
- This antibody offers improved efficiency for antigen-antibody reactions in ELISAs.
- Mab H6B11 is a valuable tool for investigating the physiological functions of plasma gelsolin.