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Updated: Sep 16, 2026
Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells
Published on: December 9, 2016
An ascites tumor appearing during the passage of Eperythrozoon coccoides in mice
Abstract:
A strain of Eperythrozoon coccoides was maintained in a pure state for 16 months in unsplenectomized Princeton mice by the intraperitoneal injection of parasitized blood, at intervals of 1 to 3 weeks. In the 39th passage three out of five mice developed marked ascites with innumerable neoplastic cells in the peritoneal fluid. By passage of this fluid the tumor cells and the eperythrozoon were transferred together, with the rapid development of ascites and early death of the animals. On continued passage of ascitic fluid and blood from the mice of the 39th successive group both the tumor and the eperythrozoon were ultimately obtained in a pure state. Development of the passaged tumor was uninfluenced by the eperythrozoon but growth of the latter appeared enhanced in the presence of the neoplastic cells. Throughout 50 passages in mice the ascites tumor has not altered in character. The intraperitoneal injection of ascitic fluid was irregularly followed by the appearance of tumor cells in the blood, the rate of the carriage being 26 per cent in sixty-five animals. The subcutaneous and intramuscular injection of the fluid resulted in solid, highly invasive, local growths which did not metastasize. The solid growths proved transferable in series yet after 20 passages still produced the ascites tumor on intraperitoneal injection of their cells.
Insights
This study maintained Eperythrozoon coccoides in mice, leading to ascites tumor development. The parasite appeared to enhance tumor growth, while the tumor did not affect the parasite.
Area of Science:
- Microbiology
- Oncology
- Parasitology
Background:
- Eperythrozoon coccoides is a microorganism that can be maintained in mice.
- Ascites tumors are a type of cancer that affects the peritoneal cavity.
Purpose of the Study:
- To investigate the long-term maintenance of Eperythrozoon coccoides in mice.
- To study the development and characteristics of an ascites tumor induced in mice.
- To explore the interaction between Eperythrozoon coccoides and the induced ascites tumor.
Main Methods:
- Maintenance of Eperythrozoon coccoides through serial intraperitoneal blood injections in mice.
- Induction and serial passage of an ascites tumor in mice.
- Co-cultivation and serial passage of the tumor and Eperythrozoon coccoides.
- Evaluation of tumor growth and parasite development under different conditions.
- Assessment of tumor cell dissemination via various injection routes.
Main Results:
- Eperythrozoon coccoides was successfully maintained in pure culture for 16 months.
- Ascites tumor developed spontaneously in mice during serial passage of Eperythrozoon coccoides.
- The parasite appeared to enhance the growth of the ascites tumor, but the tumor did not influence the parasite.
- The ascites tumor remained consistent in character over 50 passages.
- Subcutaneous and intramuscular injections led to invasive solid tumors that did not metastasize but could still induce ascites upon intraperitoneal injection.
Conclusions:
- Eperythrozoon coccoides can be maintained long-term in mice.
- A novel ascites tumor model was developed, potentially influenced by Eperythrozoon coccoides.
- The interaction suggests a potential synergistic effect favoring parasite growth.
- This model offers opportunities to study tumor biology and host-parasite interactions.
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