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Cytochrome oxidase as a redox-linked proton pump
1Department of Biochemistry and Biophysics, University of Göteborg, Sweden.
Acta Physiologica Scandinavica. Supplementum
|January 1, 1992
Summary
This study outlines general principles for redox-linked proton pumps, applying them to cytochrome oxidase. A detailed reaction scheme explains electron transfer, dioxygen reduction, and proton movements, consistent with kinetic data.
Area of Science:
- Biochemistry
- Bioenergetics
- Molecular Biology
Background:
- Cytochrome oxidase is a crucial enzyme in cellular respiration.
- Understanding its mechanism involves complex electron and proton transfer processes.
Purpose of the Study:
- To describe general principles of redox-linked proton pumps.
- To apply these principles to elucidate the mechanism of cytochrome oxidase.
Main Methods:
- Development of a reaction scheme based on redox-linked proton pump principles.
- Integration of electron transfer, dioxygen reduction, and protonation/deprotonation steps.
- Analysis of scalar and vectorial proton movements.
Main Results:
- A comprehensive reaction scheme for cytochrome oxidase is presented.
- The scheme accounts for electron transfer between redox centers.
- It details dioxygen reduction and proton translocation mechanisms.
Conclusions:
- The proposed reaction scheme is consistent with extensive kinetic data.
- This work provides a framework for understanding redox-linked proton pumps.
- It offers insights into the functional mechanism of cytochrome oxidase.