Prostanoids synthesis in lymphocyte subpopulations by adrenergic and cholinergic receptor stimulation

The release of eicosanoids (PGE2 and TXB2) as a consequence of specific neurotransmitter receptor agonist stimulation is described herein. The differential expression of beta adrenergic and muscarinic cholinergic receptors on sets and subsets of lymphocytes was first identified. Saturation assays with a specific radioligand for beta adrenergic receptors (3H-DHA) showed that B-, T-, T-helper (Th) and T-suppressor/cytotoxic (Ts/c) lymphocyte enriched populations all displayed beta adrenergic receptors. In contrast, when a specific radioligand for muscarinic cholinergic receptors (3H-QNB) was used, B-lymphocytes showed a lack of high-affinity muscarinic cholinergic receptors, while T-lymphocytes expressed them. Ts/c murine lymphocytes had more muscarinic cholinergic receptors than did Th cells. Specific receptor stimulation by the agonist caused a release of different eicosanoids depending on the cell type. Isoproterenol, triggered the release of TXB2 by B and Th-cells, but had no effect on Ts/c-cells. On the other hand, the muscarinic cholinergic agonist, carbachol only induced the release of PGE2 by Ts/c-cells. These results suggest differences in the expression and function of neurotransmitter receptors in sets and subsets of murine lymphocytes regarding the release of eicosanoids.