Japanese soybean paste miso scavenges free radicals and inhibits lipid peroxidation

Japanese soybean paste miso, which has been reported to prevent gastric and mammary cancer and chronic nephritis, was demonstrated by electron spin resonance spectrometry using 5,5'-dimethyl-1-pyrroline-N-oxide as a scavenger of free radicals. Fifty mg/ml of miso scavenged 100% of 1,1-diphenyl-2-picrylhydrazyl radicals (3.9 x 10(15) spins/ml); 45 mg/ml quenched 92% of hydroxyl radicals (7.9 x 10(16) spins/ml); and 50 mg/ml quenched 50% of superoxide anion (6.7 x 10(16) spins/ml). In the system of rat cerebral cortex homogenate supplemented with 2 mM each of Fe2+ and ascorbic acid, 90% and 82% of the hydrogen and carbon-centered radicals having 1.7 x 10(13) spins/ml and 3.9 x 10(13) spins/ml, respectively, were quenched by 180 mg/ml of miso. The thiobarbituric acid-reactive substances, an index of lipid peroxidation in the brain, was inhibited by 10 mg/ml of miso. These results showed that miso acts as an antioxidant by scavenging free radicals.