Related Experiment Videos
Temperature sensitivity of herpes simplex virus type 1 is a tissue-dependent phenomenon
1Indiana University School of Medicine, South Bend Center for Medical Education, Notre Dame.
Archives of Virology
|January 1, 1992
Summary
Herpes simplex virus type 1 (HSV-1) replication is temperature-sensitive. Supraoptimal temperatures significantly reduced infectious yields in mouse central nervous system (MNS) and mouse embryo cells (MEC), with MEC being more affected.
Area of Science:
- Virology
- Cell Biology
- Neuroscience
Background:
- Herpes simplex virus type 1 (HSV-1) is a common human pathogen.
- Understanding HSV-1's response to environmental factors like temperature is crucial for virology research.
Purpose of the Study:
- To investigate the temperature sensitivity of HSV-1 replication and morphogenesis.
- To compare HSV-1 behavior in mouse central nervous system (MNS) cells versus mouse embryo cells (MEC) at different temperatures.
Main Methods:
- Primary cultures of MNS and MEC were infected with HSV-1.
- Cells were incubated at permissive (37°C) and supraoptimal (40.5°C) temperatures.
- Infectious virus yields and viral particle ultrastructure were analyzed.
Main Results:
- Supraoptimal temperature significantly reduced HSV-1 infectious yields in both MNS and MEC.
- MEC showed a greater reduction in viral yield compared to MNS cells at 40.5°C.
- Complete HSV-1 virions were absent in MEC at 40.5°C, but present in MNS cells, indicating cell-type-specific temperature effects on viral assembly.
Conclusions:
- HSV-1 replication exhibits significant temperature sensitivity.
- A notable interaction exists between cell culture type and incubation temperature affecting HSV-1 replication and assembly.
- MNS cells demonstrate greater tolerance to supraoptimal temperatures regarding HSV-1 replication compared to MEC.