Preparation and properties of an affinity support for purification of cyclic AMP receptor protein from Escherichia coli
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J E Corrie1, C Pizza, J Makwana
1National Institute for Medical Research, London, United Kingdom.
Protein Expression and Purification
|October 1, 1992
Abstract:
Reaction of cyclic AMP with 1,1'-carbonyldiimidazole produces an intermediate which reacts with primary amines to provide a stable 2'-O-carbamyl derivative. This chemistry has been used to tether cyclic AMP to a Sepharose gel. The resulting affinity support has been used to effect a simple, nondenaturing purification of cyclic AMP receptor protein from crude cell extracts.