Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lactate content and pH in muscle obtained after dynamic exercise.

K Sahlin, R C Harris, B Nylind

    Pflugers Archiv : European Journal of Physiology
    |December 28, 1976
    PubMed
    Summary

    During exhaustive exercise, muscle acidity increases with lactate and pyruvate accumulation. Muscle lactate levels remain elevated post-exercise, while pyruvate levels shift, impacting cellular energy balance.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Strength training improves muscle aerobic capacity and glucose tolerance in elderly.

    Scandinavian journal of medicine & science in sports·2015
    Same author

    Adding strength to endurance training does not enhance aerobic capacity in cyclists.

    Scandinavian journal of medicine & science in sports·2014
    Same author

    The effect of continuous and interval exercise on PGC-1α and PDK4 mRNA in type I and type II fibres of human skeletal muscle.

    Acta physiologica (Oxford, England)·2011
    Same author

    Living with children who have coeliac disease: a parental perspective.

    Child: care, health and development·2011
    Same author

    Effect of physical training on mitochondrial respiration and reactive oxygen species release in skeletal muscle in patients with obesity and type 2 diabetes.

    Diabetologia·2010
    Same author

    Maximal lipid oxidation in patients with type 2 diabetes is normal and shows an adequate increase in response to aerobic training.

    Diabetes, obesity & metabolism·2009

    Area of Science:

    • Exercise Physiology
    • Biochemistry
    • Human Physiology

    Background:

    • Muscle metabolism during exercise is crucial for understanding energy production and fatigue.
    • Lactate and pyruvate are key indicators of anaerobic metabolism and acid-base balance.
    • Dynamic exercise elicits different metabolic responses compared to isometric exercise.

    Purpose of the Study:

    • To investigate changes in muscle and blood lactate, pyruvate, and pH during and after dynamic exercise.
    • To analyze the relationship between muscle metabolites and pH changes.
    • To examine the recovery kinetics of muscle metabolites and the NADH/NAD ratio.

    Main Methods:

    • Muscle biopsies from the quadriceps femoris were taken at rest, immediately after exercise, and during 20 minutes of recovery.

    Related Experiment Videos

  • Venous blood and capillary blood samples were analyzed for lactate, pyruvate, and pH.
  • Lactate dehydrogenase equilibrium was used to calculate NADH/NAD ratios.

    • Main Results:

    • Muscle pH significantly decreased from rest (7.08) to exhaustion (6.60), linearly correlating with muscle lactate + pyruvate.
    • Muscle lactate levels were 2-3 times higher than in blood post-exercise, decreasing with a half-time of 9.5 minutes during recovery.
    • Muscle pyruvate levels showed a slight increase, while the NADH/NAD ratio remained elevated throughout the 20-minute recovery period.

    Conclusions:

    • Dynamic exercise leads to significant muscle acidification, linked to lactate accumulation.
    • Metabolic differences observed between dynamic and isometric exercise suggest acid-base exchange with blood.
    • Impaired recovery of the NADH/NAD ratio indicates sustained alterations in cellular redox state post-exercise.