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Low density lipoprotein oxidation by stimulated neutrophils and ferritin

D S Abdalla1, A Campa, H P Monteiro

  • 1Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brazil.

Atherosclerosis
|December 1, 1992
PubMed

Insights

Ferritin enhances low-density lipoprotein (LDL) oxidation by neutrophils, involving iron release. Antioxidants like ascorbate inhibit this process, suggesting a role in inflammation.

Area of Science:

  • Biochemistry
  • Immunology
  • Oxidative Stress

Background:

  • Low-density lipoprotein (LDL) oxidation is implicated in various pathological conditions.
  • Neutrophils play a role in inflammatory responses and can generate reactive oxygen species.
  • Ferritin, an iron-storage protein, can potentially contribute to oxidative processes.

Purpose of the Study:

  • To investigate the role of ferritin in neutrophil-mediated LDL oxidation.
  • To elucidate the mechanisms involved in ferritin-enhanced LDL oxidation.
  • To assess the impact of antioxidants and other molecules on this process.

Main Methods:

  • Human neutrophils were stimulated using phorbol myristate acetate (PMA) and formylmethionylleucylphenylalanine (FMLP).
  • LDL oxidation was measured in the presence and absence of ferritin and other agents.
  • The effects of antioxidants (ascorbate, superoxide dismutase, uric acid) and other compounds (ceruloplasmin, methionine, catalase) were evaluated.

Main Results:

  • Ferritin significantly enhanced neutrophil-mediated LDL oxidation by 70%.
  • Iron released from ferritin by superoxide anions was identified as a key factor in LDL oxidation.
  • Ascorbate, superoxide dismutase, and uric acid demonstrated inhibitory effects on LDL oxidation.

Conclusions:

  • Neutrophil-mediated LDL oxidation is potentiated by ferritin, likely through iron release.
  • Antioxidants can mitigate ferritin-induced LDL oxidation.
  • These findings suggest a potential role for neutrophil- and ferritin-mediated LDL oxidation in inflammatory conditions.

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