Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fungal 17 beta-hydroxysteroid dehydrogenase.

T Lanisnik1, M Zakelj-Mavric, I Belic

  • 1Institute of Biochemistry, Medical Faculty, University of Ljubljana, Slovenia.

FEMS Microbiology Letters
|November 15, 1992
PubMed
Summary

Fungal steroid metabolism involves 17 beta-hydroxysteroid dehydrogenase (HSDH) enzymes, found in all tested species. These enzymes primarily act on the C17 steroid position, with oxidative pathways often preferred.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Involvement of the cell wall ofNocardia restricta on the bioconversions of steroid sapogenins.

World journal of microbiology & biotechnology·2014
Same author

Device for reflective dividing of a laser beam with adjustable distance between the two focus spots.

Applied optics·2010
Same author

Predicting the anti-hypertensive effect of nitrendipine from plasma concentration profiles using artificial neural networks.

Computers in biology and medicine·2005
Same author

Expression of 17beta-hydroxysteroid dehydrogenases in mesophilic and extremophilic yeast.

Steroids·2000
Same author

Characterization of fungal 17beta-hydroxysteroid dehydrogenases.

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology·2000
Same author

Pluripotency of 17beta-hydroxysteroid dehydrogenase from the filamentous fungus Cochliobolus lunatus.

Steroids·2000

Area of Science:

  • Mycology
  • Biochemistry
  • Enzymology

Background:

  • Steroid metabolism is crucial in many organisms.
  • Hydroxysteroid dehydrogenases (HSDHs) play key roles in steroid transformations.
  • Fungal HSDH activity and its specific roles remain underexplored.

Purpose of the Study:

  • To investigate the presence and characteristics of 17 beta-hydroxysteroid dehydrogenase (HSDH) activity in diverse fungal species.
  • To determine the regioselectivity and pathway preference (oxidative vs. reductive) of fungal HSDHs.
  • To explore the potential ecological or metabolic significance of HSDH in fungi.

Main Methods:

  • Testing constitutive 17 beta-HSDH activity in fungal isolates from various taxonomic classes.
  • Assessing enzyme regioselectivity for the C17 position of steroid substrates.
  • Detecting 20 beta-HSDH activity in specific fungal strains.
  • Comparing the rates of oxidative and reductive pathways catalyzed by the enzyme.

Main Results:

  • Constitutive 17 beta-HSDH activity was detected in all tested fungal representatives across different classes.
  • The enzyme exhibited regioselectivity for the C17 position of the steroid ring.
  • 20 beta-HSDH activity was specifically identified in *Trichoderma viride*.
  • The oxidative pathway was favored over the reductive pathway in most fungal species examined.

Conclusions:

  • Fungi possess widespread 17 beta-HSDH activity, suggesting a conserved role in steroid processing.
  • Fungal HSDHs are primarily C17-acting enzymes, with potential for specific isoforms like 20 beta-HSDH.
  • The prevalence of the oxidative pathway indicates its importance in fungal steroid biotransformation.

Related Experiment Videos