Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interlocking of plasmid DNAs due to Lac repressor-operator interaction.

H Y Wu1, K Lau, L F Liu

  • 1Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201.

Journal of Molecular Biology
|December 20, 1992
PubMed
Summary

The lac repressor protein can cause plasmid DNA molecules to interlock within E. coli. This DNA interlocking suggests protein interactions and may occur before daughter plasmids separate.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Research on the evaluation of the automatic construction effectiveness of a flexible point cloud deformation algorithm for nasal prostheses].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2026
Same author

Search for Light Pseudoscalar Bosons, Pair-Produced in Higgs Boson Decays in the Four-Electron Final State in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

First Evidence for Mixing-Induced CP Violation in B_{s}^{0}→J/ψϕ(1020) Decays in pp Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

Observation of Suppressed Charged-Particle Production in Ultrarelativistic Oxygen-Oxygen Collisions.

Physical review letters·2026
Same author

Measurement of D^{0} Meson Photoproduction in Ultraperipheral Heavy Ion Collisions.

Physical review letters·2026
Same author

[Sequential optimization of chromogenic staining order for a novel green substrate in immunohistochemistry double staining].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2026

Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • The lac repressor is a protein that regulates gene expression in E. coli.
  • Plasmid DNA molecules are extrachromosomal DNA found in bacteria.
  • Interlocking of DNA molecules can occur through various mechanisms.

Purpose of the Study:

  • To investigate the role of the lac repressor in mediating the formation of interlocked plasmid DNA dimers.
  • To explore the mechanism by which the lac repressor facilitates DNA interlocking in vivo.
  • To determine if lac repressor binding influences plasmid DNA segregation.

Main Methods:

  • Utilizing plasmid DNAs with a single lac repressor binding sequence in E. coli.
  • Observing the formation of homo- and hetero-interlocked dimers.

Related Experiment Videos

  • Analyzing the potential for simultaneous binding of the lac repressor to replicated plasmid molecules.

    • Main Results:

    • A single lac repressor binding sequence mediates the formation of interlocked plasmid DNA dimers.
    • Both homo- and hetero-interlocked dimers were observed, indicating random association of plasmids.
    • Multiply intertwined dimers suggest simultaneous binding to replicated daughter plasmids before segregation.
    • The formation of interlocked plasmids indicates in vivo interaction between DNA-bound proteins.

    Conclusions:

    • The lac repressor complex can facilitate gyrase-mediated interlocking of plasmid DNA molecules.
    • The lac repressor may play a role in the coordination of replicated plasmid segregation.
    • Interlocked plasmid formation serves as an indicator of protein-DNA interactions within living cells.