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Related Experiment Videos

Na channel kinetics remain stable during perforated-patch recordings.

D J Wendt1, C F Starmer, A O Grant

  • 1Department of Medicine, Duke University Medical Center, Durham, North Carolina 27706.

The American Journal of Physiology
|December 1, 1992
PubMed
Summary

The perforated-patch technique offers stable sodium channel kinetics for cardiac studies, overcoming limitations of conventional whole-cell voltage-clamp methods. This method preserves intracellular conditions, enabling reliable analysis of sodium channel function.

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Area of Science:

  • Electrophysiology
  • Cardiac Physiology
  • Ion Channel Biophysics

Background:

  • Whole-cell voltage-clamp studies of cardiac sodium channels are hindered by time-dependent shifts in gating kinetics, attributed to intracellular dialysis.
  • These shifts complicate the interpretation of sodium channel function modulation, impacting research on neurotransmitter and hormone effects.

Purpose of the Study:

  • To compare the conventional whole-cell and perforated-patch techniques for measuring cardiac sodium (Na) channel function in rabbit atrial myocytes.
  • To assess the stability of Na channel gating parameters and current-voltage relationships using both electrophysiological methods.

Main Methods:

  • Whole-cell Na currents were recorded from rabbit atrial myocytes at 17°C using both conventional whole-cell and perforated-patch voltage-clamp techniques.

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  • Steady-state inactivation and peak current-voltage relationships were measured and compared between the two techniques.

    • Main Results:

    • The perforated-patch technique demonstrated stable Na channel kinetics for up to 150 minutes, unlike the conventional method which showed shifts to more negative potentials.
    • The potential for half Na channel inactivation was -73 ± 5.1 mV with the perforated-patch technique, aligning with indirect measurements.
    • The perforated-patch method maintained the intracellular milieu, preventing dialysis-induced alterations.

    Conclusions:

    • The perforated-patch technique provides stable and reliable measurements of cardiac Na channel function, overcoming the limitations of the conventional whole-cell method.
    • This technique is advantageous for studying the modulation of Na current by various agents, as it preserves the intracellular environment.