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Related Experiment Videos

Decrease in beta 1- and increase in beta 2-adrenoceptors in long-term follow-up after orthotopic cardiac

M Steinfath1, H von der Leyen, A Hecht

  • 1Pharmakologisches Kerninstitut, Universitäts-Krankenhaus-Eppendorf, Universität Hamburg, Germany.

Journal of Molecular and Cellular Cardiology
|October 1, 1992
PubMed
Summary

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After heart transplantation, the transplanted heart shows increased beta 2-adrenoceptor density over time. This shift in beta-adrenoceptor subtypes may enhance the effects of circulating catecholamines.

Area of Science:

  • Cardiology
  • Pharmacology
  • Transplantation Biology

Background:

  • Orthotopic cardiac transplantation leads to surgical denervation of the heart.
  • Adrenergic receptor density and subtype distribution are crucial for cardiac function and response to catecholamines.

Purpose of the Study:

  • To investigate the long-term changes in beta 1- and beta 2-adrenoceptor subtype distribution in human cardiac allografts.
  • To determine if the surgically denervated, transplanted heart exhibits altered adrenoceptor profiles over time.

Main Methods:

  • Right ventricular biopsies were analyzed from 100 heart transplant recipients (1-60 months post-transplant) and 8 donor hearts.
  • Radioligand binding assays using (-)-[125I]-iodocyanopindolol (ICYP) assessed total beta-adrenoceptor density.

Related Experiment Videos

  • Selective beta 1-antagonist CGP 20712A determined beta 1- and beta 2-subtype proportions.

    • Main Results:

    • Donor hearts had a beta 1:beta 2 ratio of approximately 80:20%.
    • Total beta-adrenoceptor density remained stable until 36 months post-transplant, with a slight decrease at 48 and 60 months.
    • A significant shift in the beta 1:beta 2 ratio (to 61:39% by 60 months) occurred due to increased beta 2- and decreased beta 1-adrenoceptor numbers.

    Conclusions:

    • The transplanted human heart demonstrates a significant up-regulation of beta 2-adrenoceptors during long-term follow-up.
    • This beta 2-adrenoceptor upregulation may reflect an increased reliance on circulating catecholamines for cardiac modulation post-transplantation.