[Direct in vivo gene introduction into rat kidney]

We established a simple and efficient method in vivo gene transfer using Sendai virus (HVJ) and liposomes. Plasmid DNA and high mobility group 1 (MHG1) protein were co-encapsulated in liposomes and co-introduced into target cells by HVJ-mediated membrane fusion. pAct-SVT DNA, as a reporter gene, was introduced into the kidney of intact rats through a cannula in the renal artery, and SV 40 large T antigen was detected by enzyme immunohistochemically in glomerular cells (mesangial cells and/or capillary cells) 4 days after it's introduction. Thereafter expression of SV 40 large T antigen gene decreased and was not detected on day 10. This gene delivery system could be useful in research and development of gene therapy of renal disease.