Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Catalysis by site-specific recombinases.

W M Stark1, M R Boocock, D J Sherratt

  • 1Department of Genetics, University of Glasgow, UK.

Trends in Genetics : TIG
|December 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromosome segregation.

Current opinion in microbiology·2001
Same author

A model for the gamma delta resolvase synaptic complex.

Molecular cell·2001
Same author

Interplay between recombination, cell division and chromosome structure during chromosome dimer resolution in Escherichia coli.

Molecular microbiology·2001
Same author

Escherichia coli strains that allow antibiotic-free plasmid selection and maintenance by repressor titration.

Nucleic acids research·2001
Same author

FtsK functions in the processing of a Holliday junction intermediate during bacterial chromosome segregation.

Genes & development·2000
Same author

Resolution of holliday junctions by RuvABC prevents dimer formation in rep mutants and UV-irradiated cells.

Molecular microbiology·2000

Site-specific recombination uses recombinase enzymes to precisely rearrange DNA. These enzymes fall into two families with distinct catalytic mechanisms for DNA manipulation.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Site-specific recombination facilitates controlled DNA rearrangements.
  • Recombinase enzymes catalyze these essential DNA processes.
  • Recombinases are classified into two main families based on sequence homology.

Purpose of the Study:

  • To elucidate the current understanding of how recombinase enzymes catalyze site-specific recombination.
  • To differentiate the catalytic mechanisms employed by the two major recombinase families.

Main Methods:

  • Analysis of amino acid sequence homology to classify recombinases.
  • Investigating the catalytic mechanisms of recombinase-mediated DNA recombination.

Main Results:

Related Experiment Videos

  • Recombinases are classifiable into two distinct families.
  • The catalytic mechanisms of these two recombinase families differ significantly.
  • Detailed understanding of DNA cutting and rejoining processes.

Conclusions:

  • The two recombinase families utilize divergent strategies for catalyzing DNA recombination.
  • Understanding these mechanisms is key to controlling DNA rearrangements.