Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium calmodulin dependent kinase II in cat visual cortex and its development.

W G Jia1, C Beaulieu, Y L Liu

  • 1Department of Ophthalmology, University of British Columbia, Vancouver, Canada.

Developmental Neuroscience
|January 1, 1992
PubMed
Summary

Calcium/calmodulin-dependent protein kinase II (CAM-K II) expression is developmentally regulated in kitten visual cortex. Its levels are influenced by visual input, suggesting regulation by cortical input.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deep Learning-Generated Synthetic MR Imaging STIR Spine Images Are Superior in Image Quality and Diagnostically Equivalent to Conventional STIR: A Multicenter, Multireader Trial.

AJNR. American journal of neuroradiology·2023
Same author

Future intensification of extreme Aleutian low events and their climate impacts.

Scientific reports·2021
Same author

Bremia lactucae Infection Efficiency in Lettuce is Modulated by Temperature and Leaf Wetness Duration Under Quebec Field Conditions.

Plant disease·2019
Same author

A ZPR1 mutation is associated with a novel syndrome of growth restriction, distinct craniofacial features, alopecia, and hypoplastic kidneys.

Clinical genetics·2018
Same author

Concurrent use of capecitabine with radiation therapy and survival in breast cancer (BC) after neoadjuvant chemotherapy.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2018
Same author

[Risk factors related to HIV new infections among men who have sex with men in a cohort study].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2018

Area of Science:

  • Neuroscience
  • Developmental Neuroscience
  • Molecular Neuroscience

Background:

  • Calcium/calmodulin-dependent protein kinase II (CAM-K II) is crucial for synaptic plasticity.
  • Understanding the developmental expression of CAM-K II in the visual cortex is key to comprehending neural circuit maturation.

Purpose of the Study:

  • To investigate the developmental expression pattern of CAM-K II's alpha-subunit in the kitten visual cortex.
  • To explore the influence of early visual pathway lesions on CAM-K II expression.

Main Methods:

  • Monoclonal antibody against the alpha-subunit of CAM-K II used for visualization via immunohistochemistry.
  • Electron microscopy (EM) to determine subcellular localization.
  • Unilateral thalamic lesions in early life to remove geniculate input.

Related Experiment Videos

Main Results:

  • CAM-K II expression initiated in deep cortical layers (V, VI) and spread to other layers within 2 weeks.
  • Immunoreactivity declined in specific layers by 30-40 days, then stabilized into an adult laminar pattern.
  • EM revealed CAM-K II in both pre- and postsynaptic locations.
  • Early thalamic lesions doubled immunopositive neurons in certain layers, indicating input-dependent regulation.

Conclusions:

  • CAM-K II expression in visual cortical neurons is developmentally regulated.
  • Cortical input significantly influences CAM-K II levels, suggesting a role in activity-dependent circuit refinement.