Hydroxyl radical generation in the NADH/microsomal reduction of vanadate

ESR spin trapping measurements demonstrate generation of hydroxyl (.OH) radical from reduction of vanadate by rat liver microsomes/NADH without exogenous H2O2. Catalase decreases the .OH signal while increasing a vanadium (4+) signal. Addition of superoxide dismutase (SOD) or measurements under an argon atmosphere show decreased .OH radical production. The results suggest that during the one-electron vanadate reduction process by microsomes/NADH, molecular oxygen is reduced to H2O2, which then reacts with vanadium (4+) to generate .OH radical via a Fenton-like mechanism.