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High performance liquid chromatography of nucleic acids and the related compounds on a fluorinated silica gel column
H Itoh1, T Kinoshita, N Nimura
1School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Nucleic Acids Symposium Series
|January 1, 1992
Abstract:
Separations of nucleic acids and the related compounds were investigated by HPLC on a new fluorinated bonded silica gel column. Polyadenylate (Poly (A)) enzymatic partial hydrolysate sample and the mixture of various polynucleotide samples were sufficiently separated by the reversed-phase mode using a gradient elution with aqueous ammonium acetate/acetonitrile system. Mixed-mode separation on the fluorinated bonded phase coated with a tert-alkylammonium salt was also examined for the separation of the various polynucleotides including tRNAs.