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Immobilization of nucleoside on silica gel and specific separation of oligonucleotides
Y Inaki1, K Matsukawa, H Kawano
1Faculty of Engineering, Osaka University, Japan.
Nucleic Acids Symposium Series
|January 1, 1992
Abstract:
Deoxyadenosine was immobilized on silica gel, in order to use as HPLC resins for selective separation of oligonucleotides. The longest retention time was observed for the complementary pd(T)4, and increased with decrease of temperature. This fact suggested that the main separation factor was the base pairing between complementary nucleic acid bases. These resins may be useful for separation of components of nucleic acids and polynucleotides as a specific separation system.