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Supports for enzyme immobilization.

H C Trevisan1, L H Mei

  • 1Faculdade de Engenharia Química, Departamento de Ciência e Tecnologia de Materiais, Universidade Estadual de Campinas, SP, Brazil.

Anais Da Academia Brasileira De Ciencias
|June 1, 1992
PubMed
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Alkylamine derivatives of silica, diatomaceous earth, and glass support enhanced enzyme immobilization. Immobilized glucose oxidase showed improved pH range and thermal stability compared to free enzyme.

Area of Science:

  • Biochemistry and Materials Science
  • Enzyme immobilization and biocatalysis

Background:

  • Enzyme immobilization is crucial for industrial biocatalysis, requiring suitable support materials.
  • Porous materials like silica gel, diatomaceous earth, and glass offer potential as enzyme supports.

Purpose of the Study:

  • To evaluate alkylamine derivatives of macroporous silica gel, diatomaceous earth (celite 545), and nonporous glass as supports for enzyme immobilization.
  • To assess the suitability of these modified materials for immobilizing glucose oxidase.
  • To compare the performance of immobilized glucose oxidase with the free enzyme.

Main Methods:

  • Pore size analysis of silica gel, diatomaceous earth, and glass using mercury intrusion porosimetry.
  • Modification of support materials with alkylamine derivatives.

Related Experiment Videos

  • Immobilization of glucose oxidase onto the modified supports.
  • Enzyme activity assays to compare immobilized and free enzyme performance.
  • Evaluation of immobilized enzyme properties including optimal pH range and thermal stability.

    • Main Results:

    • Alkylamine derivatives of the tested materials were suitable for glucose oxidase immobilization.
    • The immobilized glucose oxidase demonstrated a broader optimal pH range compared to the free enzyme.
    • Enhanced thermal stability was observed for the immobilized enzyme.
    • Particle size of the carrier influenced the effectiveness of the immobilized enzyme.

    Conclusions:

    • Alkylamine-modified porous materials, including silica gel and diatomaceous earth, are effective supports for enzyme immobilization.
    • Immobilization using these supports significantly improves enzyme stability and operational pH range.
    • These findings highlight the potential of tailored materials for developing robust biocatalysts.