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[erb-B oncogene in human oral squamous cell carcinomas]
Hua Xi Yi Ke Da Xue Xue Bao = Journal of West China University of Medical Sciences = Huaxi Yike Daxue Xuebao
|September 1, 1992
Summary
The erb-B oncogene, similar to epidermal growth factor receptor (EGFr), is amplified and over-expressed in human oral squamous cell carcinomas. Gene rearrangements in erb-B may activate this proto-oncogene in tumors.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The erb-B gene, an oncogene originating from avian leukosis virus, shares similarities with the epidermal growth factor receptor (EGFr).
- Aberrant expression of oncogenes is a hallmark of various cancers, including oral squamous cell carcinomas.
Purpose of the Study:
- To investigate the amplification and over-expression of the erb-B gene in human oral squamous cell carcinomas.
- To determine the correlation between erb-B alterations and oral tumors versus other head and neck tumors.
Main Methods:
- DNA and RNA dot blot hybridization were employed to detect erb-B gene amplification and expression.
- Southern blotting analysis was used to identify potential rearrangements in the erb-B gene within tumor DNA.
Main Results:
- Amplification and over-expression of erb-B were detected in human oral squamous cell carcinomas.
- A significant correlation was found between erb-B alterations and oral epidermoid tumors.
- No correlation was observed between erb-B and non-epidermoid tumors of the head and neck.
- Southern blotting revealed rearrangements in the erb-B gene in tumor genomes.
Conclusions:
- The erb-B gene is implicated in the development of human oral squamous cell carcinomas.
- Gene rearrangement represents a potential mechanism for the activation of the erb-B proto-oncogene in oral tumors.