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Comparison of modified HAVAB and ELISA for determination of vaccine-induced anti-HAV response
1SmithKline Beecham Biologicals, Rixensart, Belgium.
An inhibition ELISA was compared with a modification of the HAVAB assay for measuring antibodies induced by a killed HAV vaccine. GMT's expressed in mIU/ml were higher by ELISA than by modified HAVAB, especially after the first and second doses of vaccine but seroconversion rates were very similar and a good correlation was found between both assays. Because of its higher sensitivity and specificity, the ELISA assay was preferred to modified HAVAB for the evaluation of a Hepatitis A vaccine in human volunteers.
An inhibition ELISA was compared with a modification of the HAVAB assay for measuring antibodies induced by a killed HAV vaccine. GMT's expressed in mIU/ml were higher by ELISA than by modified HAVAB, especially after the first and second doses of vaccine but seroconversion rates were very similar and a good correlation was found between both assays. Because of its higher sensitivity and specificity, the ELISA assay was preferred to modified HAVAB for the evaluation of a Hepatitis A vaccine in human volunteers.